PCR: Basic Principles
COURSE 2025A
Participants:
Jaider Arnulfo Guzmán González
Molecule: HSP70, Leishmania brazilienzis, XM_001566275.1
Ahadini, S. N., Tyasningsih, W., Effendi, M. H., Khairullah, A. R., Kusala, M. K. J., Fauziah, I., Latifah, L., Moses, I. B., Yanestria, S. M., Fauzia, K. A., Kurniasih, D. A. A., & Wibowo, S. (1970). Molecular detection of blaTEM-encoding genes in multidrug-resistant Escherichia coli from cloacal swabs of ducks in Indonesia farms. Open Veterinary Journal. https://doi.org/10.5455/OVJ.2025.v15.i1.8
Danna Isabella Manrique Tovar
Molecule: HSP70, Ovis aries, NM_001267874.1
Azarm A, Dalimi A, Mohebali M, Mohammadiha A, Pirestani M, Zarei Z, Zahraei-Ramazani A. Molecular Identification of Leishmania infantum kDNA in Naturally Infected Dogs and Their Fleas in an Endemic Focus of Canine Visceral Leishmaniasis in Iran. J Arthropod Borne Dis. 2022 Sep 30;16(3):243-250. doi: 10.18502/jad.v16i3.12041.
Nicole Dallana García Aviles
Molecule: ACTB, Homo sapiens, NM_001101.5
Matos-Rodrigues, G. E., Masseron, C. C., Silva, F. J. M. D. A., Frajblat, M., Moreira, L. O., & Martins, R. A. P. (2020). PCR-based detection of Helicobacter spp. in animal facilities of a University in Rio de Janeiro, Brazil. Anais Da Academia Brasileira de Ciencias, 92(4), e20191517. https://doi.org/10.1590/0001-3765202020191517
Silvia Fernanda Campos Hernández
Molecule: EF-1 alpha, Sus scrofa, NM_001097418.2
Sheet, O. H. (2022). Molecular detection of mecA gene in methicillin-resistant Staphylococcus aureus isolated from dairy mastitis in Nineveh governorate, Iraq. Iraqi Journal of Veterinary Sciences, 36(4), 939–943. https://doi.org/10.33899/ijvs.2022.132643.2115
Santiago Gamboa Espinosa
Molecule: Tp53, Canis lupus familiaris, NM_001389218.1
Tan, C. Y., Thanawongnuwech, R., Arshad, S. S., Hassan, L., Lee, C. Y., Low, S. E., … & Ooi, P. T. (2023). First molecular detection of Porcine circovirus type 4 (PCV4) in Malaysia. https://doi.org/10.47665/tb.40.3.005
Dilan Paez Gutierrez
Molecule: IFNT2, Bos taurus, NM_001015511.4
Osorio-Muñoz K. & Rondón-Barragan, I. S. Molecular detection of Mycoplasma spp. and its associated risk factors in domestic cats in the city of Ibagué, Tolima. The Thai Journal of Veterinary Medicine, 54(2), 263-267. https://doi.org/10.56808/2985-1130.3724
Anderson Santiago Leyva Ardila
Molecule: CRP, Gallus gallus, NM_001039564.2
Feronato, C., Leme, R. A., Diniz, J. A., Agnol, A. M. D., Alfieri, A. F., & Alfieri, A. A. (2018). Development and evaluation of a nested-PCR assay for Senecavirus A diagnosis. Tropical animal health and production, 50, 337-344. https://doi.org/10.1007/s11250-017-1436-z.
Laura Tatiana Díaz Molina
Molecule: AQP7, Bos taurus, NM_001076378.2
Cho, J., Jeong, S., Kim, M., Cho, W. S., Kim, D., & Park, C. (2024). Loop-mediated isothermal amplification polymerase chain reaction in place of a modified Knott test in screening dogs for heartworm (Dirofilaria immitis) infection combined with antigen detection test. American Journal Of Veterinary Research, 85(9). https://doi.org/10.2460/ajvr.24.02.0027
Laura Chacón Tique
Molecule: Raf-1, Homo sapiens, NM_001354695.3
Koshino, A., Goto-Koshino, Y., Setoguchi, A., Ohno, K., & Tsujimoto, H. (2016). Mutation of p53 gene and its correlation with the clinical outcome in dogs with lymphoma. Journal of Veterinary Internal Medicine, 30(1), 223–229. https://doi.org/10.1111/jvim.13807
Natalia Chacón Tique
Molecule: AQP5, Bos taurus, NM_001191160.1
Ayene, Y. Y., Mohebali, M., Hajjaran, H., Akhoundi, B., Shojaee, S., Rahimi-Foroushani, A., Afshar, M. J. A., & Zarei, Z. (2021). A comparative study of nested-PCR and direct agglutination test (DAT) for the detection of Leishmania infantum infection in symptomatic and asymptomatic domestic dogs. BMC Research Notes, 14(1). https://doi.org/10.1186/s13104-021-05654-0
Juan David Mendoza Cañón
Molecule: 18s, Hepatozoon canis, MK757814.1
Yadav, J. P., Das, S. C., Dhaka, P., Mukhopadhyay, A. K., Chowdhury, G., Naskar, S., & Malik, S. S. (2018). Pulsed-field gel electrophoresis of enterotoxic Clostridium perfringens type A isolates recovered from humans and animals in Kolkata, India. International Journal of Veterinary Science and Medicine, 6(1), 123–126. https://doi.org/10.1016/j.ijvsm.2017.11.002
Juan David Mendez Cuellar
Molecule: GATA3, Bos taurus, NM_001076804.1
Dehuri, M., Panda, M., Sahoo, N., Mohanty, B., & Behera, B. (2021). Nested PCR assay for detection of Theileria annulata in Hyalomma anatolicum infesting cattle from coastal Odisha, India. Animal Biotechnology, 33(6), 1229-1234. https://doi.org/10.1080/10495398.2021.1885427
Catalina Lapuente Chala
Molecule: Gfap, Danio rerio, NM_131373.2
Ekawasti F, Nurcahyo RW, Nashrulloh MF, Priyowidodo D, Prastowo J. Development of a multiplex polymerase chain reaction technique for detection and discrimination of Eimeria spp. in cattle in Indonesia. Vet World. 2022 Apr;15(4):975-980. https://doi.org/doi:10.14202/vetworld.2022.975-980.
Jeison Alfonso Yaima Yate
Molecule: Plasmepsin V, Babesia bovis, 5479544
Saito, K., Kimata, K., Watahiki, M., Isobe, J., Kanatani, J. I., Ikeda, K., Takeuchi, T., Matsuzaki, C., & Oishi, K. (2025). Investigation of an Outbreak of Clostridium perfringens in Toyama, Japan, 2023 Using Single-Nucleotide Polymorphism Analysis for Genotyping. Japanese journal of infectious diseases, 78(1), 47–50. https://doi.org/10.7883/yoken.JJID.2024.189
Here you will find a supportive videographic material for the course. Please enjoy, suscribe to LIBM Youtube channel for more content.
These videographic material is associated with the Course of Applied Molecular Biology from the Laboratory of Immunology and Molecular Biology (Universidad del Tolima) Project “IDENTIFICACIÓN DE BIOTIPO BOVINO, MEDIANTE BIOTECNOLOGÍA MOLECULAR, ADAPTABLE AL CAMBIO CLIMÁTICO EN EL DEPARTAMENTO DE CÓRDOBA, CARIBE”, BPIN code 2016000100026 of the Sistema General de Regalías